Does it matter which PCIE slot you put a graphics card in?

3-slot and 16 gigabytes GPUs Video Graphics Cards · Shorter than 200mm · 251mm to 300mm · Over 300mm

The only way to add a GPU is via a PCIe x16 slot because, as mentioned previously, the majority of GPUs are designed to utilize 16 PCIe

gpu with m2 slot Tech Specs · CPU Cooler Clearance: Up to 165mm · GPU Clearance: Up to 435mm · PSU Clearance: 200mm · Cable Management: 91mm

gpu with nvme slot GPU, AMD Radeon™ HD 8870M Series GPU, AMD Radeon™ HD 8850M Series GPU, AMD Size All, 24 GB, 20 GB, 16 GB, 12 GB, 10 GB, 8 GB, 6 GB, 4 GB, 3 GB, 2 GB, 1 GB